Regulation of Sulfur Metabolism in Cephalosporium acremonium

Cephalosporium acremonium에서 황화합물 대사의 조절

  • Lee, Kyoung (Genetic Engineering Center, Korea Advanced Institute of science and Technology) ;
  • Park, Sang-Ho (Genetic Engineering Center, Korea Advanced Institute of science and Technology) ;
  • Lee, Jung-Joon (Genetic Engineering Center, Korea Advanced Institute of science and Technology) ;
  • Mheen, Tae-Ick (Genetic Engineering Center, Korea Advanced Institute of science and Technology)
  • Published : 1987.10.01

Abstract

A DL-seleno-methionine resistant mutant, Cephalosporium acremonium MS-92 showed increased activities of sulfate and L-methionine uptake than the parent strain, and accumulated excess methionine and S-adenosylmethionine (SAM) intracellularly. And the sulfate uptake system was severely inhibited by L-cysteine. In crude enzyme extracts, the mutant MS-92 showed lower L-serine sulfhydrylase (identical with cystathionine $\beta$-synthase) activity than the parent. Also, cysteine desulfhydrylase activity, an index of intracellular L-cysteine concentration, of the mutant MS-92 was decreased by about 50% as com-pared with that of the parent. Thus, it was supposed that the mutant MS-92 should have n lower level of L-cysteine than the parent. In C. acremonium like A. nidulans, the enzymes related to the biosynthesis of methionine might be regulated by L-cysteine, but not by methionine or SAM.

Keywords

Cephalosporium acremonium;Cephalosporin C(CPC);S-adenosylmethionine (SAM);Regulation;Sulfur Metabolism