A New Restriction Endonuclease from Clostridium thermocellum

Clostridium thermocellum으로 부터 새로운 type I I 제한효소 Cth I 의 분리

  • Choi, K.D. (Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology) ;
  • Kim, Kitae (Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology) ;
  • Yoo, Ook-Joon (Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology)
  • Published : 1987.10.01

Abstract

The isolation and characterization of type II restriction endonuclease from Clostridium thermocellum ATCC 27405 were described. This enzyme (Cth I endonuclease) is an isoschizomer of Bcl I endonuclease recognizing 5'-TGATCA-3'. Cth I endonuclease requires MG$^{2+}$ ion for its activity and is maximally active at PH 1.5 to 10.5 in the Presence of 0 to 10mM NaCl. Cth I endonuclease is heat stable and has an optimum temperature of 6$0^{\circ}C$. The activity of Cth I enzyme is sensitive to dam methylation.

Keywords

Type I I restriction endonuclease;Cth I;Bcl I;isoschizomer;dam methylation