Cloning and Expression in Escherichia coli of a Cellulase Gene from Clostridium thermocellum

Clostridium thermocellum의 Cellulase 유전자의 Cloning

  • 하지홍 (경북대학교 자연과학대학 유전공학과) ;
  • 한성숙 (경북대학교 자연과학대학 유전공학과) ;
  • 김욱한 (경북대학교 자연과학대학 유전공학과) ;
  • 이용현 (경북대학교 자연과학대학 유전공학과)
  • Published : 1987.10.01

Abstract

A cellulase gene of Clostridium themocellum was transferred to Escherichia coli by molecular cloning with pBR322. The gene was carried in a Hind III digested DNA sequence of about 1.8 kb. This Rind III fragment expressed activities on carboxymethyl cellulose (CMC) and on filter gaper in E. coli. The expression of clostridial cellulase gene in E. coli was studied and compared with the pro-ducts of cellulase genes in C. themocellum.

Keywords

Recombinant DNA;cellulase genes;C. themocellum;gene expression