Enzymatic Conversion of Pyruvic Acid to Tryptophan tinted to Pyruvic Acid-Producing Microorganism

Pyruvic Acid 생산 미생물과 연결된 Pyruvic Acid의 Tryptophan으로의 효소적 전환

  • 정남현 (고려대학교 농과대학 농화학과과) ;
  • 방원기 (고려대학교 농과대학 농화학)
  • Published : 1987.10.01

Abstract

Enzymatic conversion of pyruvic acid produced by microorganism to tryptophan was investigated. A luminescent bacteria. Beneckea sp., was used for the production of pyruvic acid. As a source of tryptophanase which synthesizes tryptophan from pyruvic acid, indole and ammonia, whole cells of Enterobacter aerogenes ATCC 10031 were used directly in the reaction mixture. To increase the production of tryptophan, nonionic detergents and nonaqeous organic solvents were used ms reserviors of indole in the reaction mixture. In the case of nonionic detergents, TritonX-100 was very effective. When 1.5% of Triton X-100 was used, 7.7g/$\ell$ of tryptophan was produced at 37$^{\circ}C$ for 48hr. In the case of nonaqueous solvents, 8.7g/$\ell$ of tryptophan was produced at 37$^{\circ}C$ for 48 hr, when 10% of benzene was used. This amount of tryptophan corresponds to conversion of 48% of Indole and 36% of pyruvic acid, respectively.

Keywords

Enzymatic conversion;tryptophan;Enterobacter aerogenes