Stabilities of Plasmid Vectors in Zymomonas mobilis

Zymomonas mobilis Plasmid Vector의 숙주세포 내에서의 안정성에 관한 연구

  • 이상기 (한국과학기술원 유전공학센터) ;
  • 박은숙 (한국과학기술원 유전공학센터) ;
  • 황덕주 (한국과학기술원 유전공학센터) ;
  • 박무영 (한국과학기술원 생물공학과)
  • Published : 1987.10.01

Abstract

The stabilities of plasmid vectors in Zymomonas mobilis were tested in batch and continuous cultures. It was found that the growth of the host Zymomonas strain was greatly affected by the size of plasmids as well as the composition of nutrient media: the host cells grew taster when harboring plasmids of smaller sizes and in n non-selective medium. All the Zymomonas plasmid vectors containing antibiotics selective markers and Zymomonas replication origins could be maintained in a stable manner over 30 generations without being integrated into host chromosomes.

Keywords

Zymomonas mobilis;plasmid vectors;stability;host cells