Construction of Plasmid Vectors for Zymomonas mobilis

Zymomonas mobilis의 Plasmid Vector 제조에 관한 연구

  • Hwang, Duk-Ju (Genetic Engineering Center, Korea Advanced Institute of Science and Technology) ;
  • Rhee, Sang-Ki (Genetic Engineering Center, Korea Advanced Institute of Science and Technology) ;
  • Pack, Moo-Young
  • Published : 1987.10.01

Abstract

In order to develop useful plasmid vectors for Zymomonas cells, attempts were made to isolate natural plasmids from Z. mobilis ATCC10988. Among a few plasmids isolated, a small plasmid of 3.9 Kb size was chosen and designated as pZM3. By introducing the replication origin of pZM3 into pBR325, a hybrid plasmid vector of 8.4 Kb size, pHZ22, was constructed. This vector contained chloramphenicol resistant gene as a selectable marker and proved to be conjugally transmissible and stably maintained in Z. mobilis. Tetracycline resistant gene was isolated from RP4 and introduced into pHZ22 to make a new vector called pHZT224 of 10.7 Kb size. Through n series of experiments, it was evident that these plasmid vectors containing selectable markers of chloramphenicol and tetracycline resistance were shuttle vectors functional in Z. mobilis as well as E. coli.

Keywords

plasmid vector;Z. mobilis;antibiotic markers;natural replicon;conjugation;shuttle vectors