Hydrolysis of Triglyceride in Two Phase System Using Immobilized Lipase

이상계내에서 고정화리파제에 의한 트리글리세리드의 가수분해

  • Kwon, Dae Y. (Food Research Institute, AFMC) ;
  • Kim, Kee H. (Division of Biotechnology, R & D Center) ;
  • Joon S. Rhee (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology)
  • Published : 1987.04.01

Abstract

Lipases from Candida rogosa and Rhizopus arrhizus were immobilized by entrapment with photo-crosslinkable resin prepolymer for the study of fat splitting and interesterification in isooctane-two phase system. Dioctylsulfosuccinate was selected as the most suitable surfactant during the immobilization. Lipase entrapped with hydrophobic photo-crosslinkable resin prepolymer(ENTP-3000) exhibited the highest activity, whereas lipase entrapped with hydrophilic gel(ENT-4000) was more stable in organic solvent. As the degree of hydrophobicity of the immobilization matrix was increased, Vm(app) of the lipase entrapped was increased, but Km(app) was approximately constant. While the optimum pH of the lipases entrapped on hydrophilic gel (ENT-4000) were around pH 7.0 for Candida lipase and Rhizopus lipase, the reaction rate of the lipases entrapped on hydrophobic gel were less dependent on pH variations for short reaction time. However, for longer reaction time, the lipnses from C. rugosa and R. arrhizus entrapped on hydrophobic gel yielded maximum rate at pH 6.0 and 6.5, respectively, Entrapment method endowed the lipase with thermal stability.

Keywords

Hydrolysis of triglyceride;two phase system;immobilized lipase;photo-crosslinkable resin prepolymer