Cloning of nif genes from Enterobacter agglomerans in Escherichia coli.

Enterobacter agglomerans의 질소고정유전자 Cloning

  • 정건섭 (연세대학교 식품공학과) ;
  • 이정기 (연세대학교 식품공학과) ;
  • 민태익 (한국과학기술원 유전공학센타) ;
  • 변유량 (연세대학교 식품공학과) ;
  • 유주현 (연세대학교 식품공학과)
  • Published : 1987.04.01

Abstract

In order to cloning of the nif genes of Enterobacter agglomerans NFB-264, the digested total DNA of the strain was ligated to pBR 322 and transformed into E. coli. Through the negative selection and colony hybridization, the transformants were obtained. The recombinant plasmids, pNEL 10 and pNES 20 were extracted from these transformants. It was known from Southern hybridization that pNEL 10 contained the 12 Mdal foreign DNA fragment hybridized with nif Q-X probe and pNES 20 included the 5 Mdal foreign DNA fragment hybridized with nif NE and nif YK probe.

Keywords

nif gene cloning;Enterobacter agglomerans