Intraspecific Protoplast Fusion of Brevibacterium and Intergeneric Protoplast Fusion between Brevibacterium flavum and Corynebacterium glutamicum and the Metabolic Control of L-Lysine Biosynthesis in Improved Bacterial Strains

Brevibacterium flavum의 동종간 및 Corynebacterium glutamicum과의 이속간 원형질체 융합 및 개량균주의 L-Lysine 생합성의 대사제어

  • Park, Chung (Department of Agricultural Chemistry, Korea University) ;
  • Im, Beon-Sam (Department of Agricultural Chemistry, Korea University) ;
  • Jeon, Moon-Jin (Department of Agricultural Chemistry, Korea University)
  • 박청 (고려대학교 농화학과) ;
  • 임번삼 (고려대학교 농화학과) ;
  • 전문진 (고려대학교 농화학과)
  • Published : 1987.04.01

Abstract

As a trial method of breeding L-lysine producing strains, the intraspecific protoplast fusion bet-ween Brevibacterium flavum ATCC 21528R and Brevibacterium flavum ATCC 21529S and the intergeneric protoplast fusion between Brevibacterium flavum ATCC 21528R and Corynebacterium glutamicum ATCC 13058S were performed. The optimum conditions for protoplast formation of these strains were examined and the effect of plasma expander on regeneration and/or fusion was also observed. Both fusants No. CH23 and No. CH4l showed higher productivity of L-lysine than those of parental cells under the optimum cultural conditions at a rate of 21% and 8.9%, respectively. And, activity of several enzymes in L-lysine biosynthetic pathway including aspartokinase, a rate-limiting enzyme, was determined. Besides, metabolic control mechanism of L-lysine biosynthesis in fusant No. CH23 and in No. CH41 was investigated to compare with that of parental strains.

Keywords

Protoplast fusion;L-lysine biosynthesis;metabolic control