Kinetics of Lipase Reactions in Two Phase System

이상계내에서 리파제의 반응동력학

  • Kwon, Dae Y. (Food Research Institute, AFMC) ;
  • Joon S. Rhee (Department of Biological Science and Engineering Korea Advanced Institute of Science and Technology)
  • Published : 1987.04.01


Two phase reaction system was used to hydrolyze the olive oil for fat splitting. Kinetics of lipases in two phase system were investigated by determining the hydrolysis rate of triglycerides at various olive oil concentrations in isooctane using the microbial lipases from Candida rugosa and Rhizopus arrhizus. The rate equation in lipid hydrolysis for various olive oil concentrations in two phase system was deviated from the Michaelis-Menten kinetics. The results suggested that the olive oil concentration in isooctane affects the interfacial area. The dependency of the interfacial area on olive oil concentration is greater at the lower olive oil concentration than at the higher substrate concentration. We modified the rate equation by considering the interfacial area between two phases depending on the olive oil concentration in solvent phase.


Kinetics of lipase;Two phase system;Lipase;Lipase reaction mechanism