Conditions of Protoplast Formation and Regeneration of Streptomyces mitakaensis

Streptomyces mitakaensis의 원형질체 형성 및 재생조건 연구

  • 한순옥 (건국대학교 생물학과 유전공학연구소) ;
  • 이영주 (건국대학교 생물학과 유전공학연구소) ;
  • 이형환 (건국대학교 생물학과 유전공학연구소)
  • Published : 1987.04.01

Abstract

The optimal conditions for the protoplast formation and regeneration of Streptomyces mitakaensis have been investigated. S. mitakaensis cells were converted to protoplast by treating with 0.1 mg/$m\ell$ of lysozyme in phosphate-tris buffer (pH 7.2) to the cells grown at the late logarithmic growth phase in the GBYN medium (gycerol 20g, beef extract 5g, yeast extract 5g, NaCl 5g in 1 liter of distilled water) contained 0.5% glycine. Cell regeneration from protoplast was accomplished in 10 days post inoculation on the R2 regeneration agar medium and at 3 days post inoculation on the H2 regeneration liquid medium. The efficiency of the regeneration was 0.l% in 3 days at 35$^{\circ}C$.

Keywords

Protoplast formation & regeneration;Streptomyces mitakaensis