Purification of Intracellular $\beta$-Galactosidase from Lactobacillus sporogenes in an Aqueous Poly(ethylene glycol)- Potassium Phosphate Two-Phase System

Poly(ethylene glycol)/인산염 용액 2상계를 이용한 Lactobacillus sporogenes가 생산하는 균체내 $\beta$-Galactosidase의 추출 분리에 관한 연구

  • 이삼빈 (고려대학교 농과대학 식품공학과) ;
  • 김영만 (일동제약㈜) ;
  • 이철호 (고려대학교 농과대학 식품공학과)
  • Published : 1987.04.01

Abstract

Poly(ethylene glycol)-PPB two phase system was used tot the purification of $\beta$-galactosidase from Lactobacillus sporogenes. The smaller the molecular weight of concentration of PEG phase in-creased, proteins as well as $\beta$-galactosidase was partitioned into the top phase. All cell debris were confined to the potassium phosphate phase (bottom phase), approached to the binodial line. The purification ratio increased by changing the polymer-salt composition of the tie line towards higher salt concentrations. It was also possible to obtain higher purification of the enzyme after two-step extraction using PEG 1000 and PEG 300. The top phase contained 74% of the total $\beta$-galactosidase with a purification factor of 2.1.

Keywords

$\beta$-Galactosidase;Lactobacillus sporogenes;two-phase system