Reverse Reaction of L-Phenylalanine Ammonia-Lyase derived from Rhodotorula glutinis for the Production of L-Phenylalanine

Rhodotorula glutinis의 L-Phenylalanine Ammonia-Lyase의 역반응을 이용한 L-Phenylalanine 생성

  • Kang, Bong-Kyung (Department of Life Science, Pacific R&D Center) ;
  • Park, Jin-Young (Department of Life Science, Pacific R&D Center) ;
  • Kiomin Chung (Department of Life Science, Pacific R&D Center)
  • Published : 1987.04.01

Abstract

During the enzymatic production of L-phenylalanine exploiting L-phenylalanine ammonia lyase(E.C 4.3.1.5) and trans-cinnamic acid, the conversion yield of L-phenylalanine and the stability of L-phenylalanine ammonia-lyase per so or induced Rhodotorula glutinis IFO 0559 were investigated. And the glycerol added to the conversion reaction as stabilizer had effect only on L-phenylalanine and made it possible to obtain the 80% conversion yield from trans-cinnamic acid. In addition, the more rapid and reliable method than the thin layer chromatography for determining the conversion yield will be disscused.

Keywords

L-Phenylalanine ammonia lyase;L-phenylalanine;trans-cinnamic acid