A Novel saccharification method of uncooked concentrated corn starch using an agitated bead reaction system

분쇄마찰매체 함유 반응계를 이용한 무증자 Corn starch의 고농도 당화와 당화액의 조성에 관한 연구

  • 이용현 (경북대학교 자연과학대학 유전공학과) ;
  • 조구형 (경북대학교 자연과학대학 유전공학과)
  • Published : 1986.10.01

Abstract

Corn starch was saccharified without cooking in an agitated bead reaction system. Uncooked corn starch was effectively hydrolyzed even at the concentration as high as 39%(w/v). After 24 hours. the extent of saccharification reached at 92%, which corresponds glucose concentration of 425g/L. Fed-batch feeding of starch was more effective than batch feeding for saccharification of uncooked corn starch. The composition of hydrolysated of uncooked starch was analyzed. which was composed of 95% glucose, 0.7% of maltose, and 4.5% of high saccharide, similar with that of cooked starch. The hydrolysate can be successfully utilized for HFCS manufacture. The starch liquefying and saccharifying enzyme was relatively stable even be the physical impact of the attrition-milling media. The enzyme stabilizer, $Ca^{++}$, played an essential role in preventing the enzyme deactivation caused by the physical impact.