A study on strain improvement by protoplast fusion between amylase secreting yeast and alcohol fermenting yeast - III. Isolation and characterization of fusant between S. diastaticus and C. tropicalis

Amylase분비효모와 alcohol발효효모의 세포융합에 의한 균주의 개발 - 제3보. S. diastaticus와 C. tropicalis 간의 세포융합 및 융합체의 성질-

  • 서정훈 (경북대학교 자연과학대학 미생물학과) ;
  • 권택규 (경북대학교 자연과학대학 미생물학과) ;
  • 홍순덕 (경북대학교 자연과학대학 미생물학과)
  • Published : 1986.10.01

Abstract

S. diastaticus hydrolysised $\alpha$-1.4 linkage of the starch and was known fermenting yeast strain, but poorly hydrolized $\alpha$-1.6 linkage of the starch. To improve the starch fermentation ability of yeast, we tried that protoplast fusion between S. diastaticus and C. tropicalis and finally two starins of fusant (FPDC42, FPDC43) were obtained. C. tropicalis well hydrolysis both $\alpha$-1.4 and $\alpha$-1.6 linkanges in the starch. The protoplast of parental auxotrophic cells were fused in the presence of 10mM CaCl$_2$ and 35% of polyethylene glycol (M. W. 4,000). The fusion frequency was 10$^{-5}$ to 10$^{-6}$. Properties of the fusants(genetic stability, assimilation of carbon sources, random spore formation, copper resistance, NaCl tolerance, DNA content, cell size and growth rate) were investigated.

Keywords

S. diastaticus;C. tropicalis;glucoamylase;protoplast fusion