Properties of Extracellular Polyphenol Oxidase Isolated from Lentinus edodes JA01

Lentinus edodes JA01에서 분리한 세포외 polyphenol oxidase의 부분적 성질에 관하여

  • 신광수 (서울대학교 자연과학대학 미생물학과) ;
  • 김규중 (강릉대학 생물학과) ;
  • 홍순우 (서울대학교 자연과학대학 미생물학과) ;
  • 하영칠 (서울대학교 자연과학대학 미생물학과)
  • Published : 1986.06.01


To find the role of polyphenol oxidase in lignin biodegradation, chracteristics of extracellular polyphenol oxidase activity from Lentinus edodes JA01 was investigated. Polyphenol oxidase had its optimum activity at pH 4.5 and $45^{\circ}C$ respectively. Also, the enzyme was very unstable in various pHs and comparatively heat stable up to $60^{\circ}C$. In $lignosulfonate-NH_4$ salts medium, the growth rate of L.edodes JA 01 was relatively slow and polyphenol oxidase activity appeared 2 and 14 days after inoculation. No significant relationships were found between polyphenol oxidase activity and the amounts of lignosulfonate present in the culture medium.


lignosulfonate-$NH_4$ salts;polyphenol oxidase;Lentinus edodes