Isolation of Salicylate-Degrading Plasmid from Pseudomonas putida

Pseudomonas putida로 부터 salicylate 분해 plasmid의 분리

  • 배경숙 (서울대학교 자연과학대학 미생물학과) ;
  • 나종욱 (서울대학교 자연과학대학 미생물학과) ;
  • 강사욱 (서울대학교 자연과학대학 미생물학과) ;
  • 홍순우 (서울대학교 자연과학대학 미생물학과) ;
  • 하영칠 (서울대학교 자연과학대학 미생물학과) ;
  • 이계준 (서울대학교 자연과학대학 미생물학과)
  • Published : 1986.06.01

Abstract

The large plasmid (about 180 megadaltons) was isolated from the aquatic strain of Pseudomonas which was found to degrade salicylate. It was found that the plasmid could be isolated under gentle conditions in comparison with other methods. The yield of covalently closed circular DNA was enganced by heat treatment at $55^{\circ}C$ after denaturing the chromosomal DNA with alkaline sodium dodecyl sulfate (pH 12.45), and the plasmid DNA was selectively concentrated by utilizing 10% polyethylene glycol as final concentration. It was also found that the cured strains with mitomycin C did not show any growth on the medium containing salicylat6e, therefore, it was concluded that the plasmid might play and important role on the salicylate degradation.

Keywords

plasmid;Pseudomonas putida;salicylate;curing