Studies on the Oranization and Expression of tRNA Genes in Aspergillus nidulans (V) The Molecular Structure of $tRNA^{Arg}$ in Aspergillus nidulans

Aspergillus nidulans의 tRNA유전자의 구조와 발현에 관한 연구 V Aspergillus nidulansd의 $tRNA^{Arg}$ 분자구조

  • 이병재 (서울대학교 자연과학대학 미생물학과) ;
  • 강현삼 (서울대학교 자연과학대학 미생물학과)
  • Published : 1986.06.01

Abstract

We have determined the sequence of $tRNA^{Arg}$ of A. nidulans partially by enzymatic rapid RNA sequencing technique. The sequence was 5'GGCCGGCUGGCCCAAXUGGCAAGGXUCUGAXUACGAAXCAGGAGAUUGCACXXXXXGAGCXXUXXGUCGGUCACCA3' The cloverleaf structure was made from above data. As a result, the anticodon sequence was identified as ACG. This result was confirmed with charging test. The complete sequence was proposed by supplementing the DNA sequence to and by assigning the position of minor bases to this RNA sequence.

Keywords

$tRNA^{Arg}$ gene;DNA sequencing;consensus sequences