Effect of Sodium deoxycholate and Sodium dodecy sulfate on Phospholipid Composition and Phospholiases of Rhizopus oryzae

Rhizopus oryzae의 인지질과 그 분해효소에 미치는 계면활성제의 영향

  • 윤희주 (한양대학교 생물학과) ;
  • 조기승 (한양대학교 생화학과) ;
  • 최영길 (한양대학교 생물학과)
  • Published : 1986.03.01


Effect of sodium deoxycholate and sodium dodecyl sulfate on Rhizopus oryzae were investigated. Morphological change was obtained by supplement of these surfactants into culture media during the sumerged culture. In accordance with morphological changes, composition of phospholipid was changed. In case of surfactant-free culture, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine were measured more than 95% of total phospholipid. But cardiolipin and phosphatidylinositol were conspicuously increased by treatment of both sufactants. Presence of phospolipase A, C, and D were detected from mycelium. Phospholipase A and D were activated by supplement of sodium deoxycholate and C was activated by sodium dodecyl sulfate. These results were interpreted in respect of polymorphism of phospholipid and membrane stability against solubilization effect of surfactants.