Characterization of the Restriction Endonuclease Bki I from Brevibacterium divaricatum

Brevibacterium divaricatum의 제한효소 Bdi I의 특성

  • 김용석 (서울대학교 자연과학대학 동물학과) ;
  • 노현모 (서울대학교 자연과학대학 동물학과)
  • Published : 1986.03.01

Abstract

A new type II restriction endonuclease, Bdi I, has been isolated from Brenibacterium divaricatum FERM 5948 by procedures of ammonium sulfate fractionation, DEAE-cellulose chromatography and heparin agarose chromatography. The purified Bdi I restriction endonudlease had the same cleavage patterns of Cla I whose recognition sequence is 5' ATCGAT 3'. From the result that ${\lambda}-Cla$ I DNA frahment could be cloned in pBR 322 digested with Bdi I, it has been proven that Bdi I cuts between T and C(5' AT/CGAT3') within the recognition sequence and produces 5'pCG cohesive end. The optimal temperature for the Bdi I restriction endonuclease activity was $37^{\circ}C$, and optimal salt (NaCl) concentration was 50-100 mM.

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