Restriction map of a cryptic plasmid from Pseudomonas putida

Pseudomonas putida로 부터 분리한 cryptic플라스미드의 제한효소지도

  • 김훈규 (고려대학교 이과대학 생물학과) ;
  • 고상균 (고려대학교 이과대학 생물학과) ;
  • 이영록 (고려대학교 이과대학 생물학과)
  • Published : 1986.03.01


We screened lysates of the laboratory strains of pseudomonads utilizing hydrocarbon by agarose gel electrophoresis and cesium chloride-ethidium bromide equilibrium centrifugation, to find an intrinsic plasmid as a vector and to examine the relationship between the plasmid and hydrocarbon degradation. Only one strain from the examined strains, Pseudomonas putida KU190, contained a plasmid. We named the plasmid pKU41. The molecular size of pKU41 was determined as 41kb, using covalently closed circular forms of RP4 and pSY343 as standard size markers. The restriction sites of pKU41 for BamHI, BglII, EcoRI, HindIII, and SalI were 3, 1, 3, 6 and more than 13, respectively. With double or triple digestion, restriction map of pKU41 was constructed for BamHI, BglII and HindIII. For elucidation on the biological function of the plasmid, test was conducted on the ability of hydrocarbon utilization of the host strain but no apparent relationship was observed.