Isolation and identification of tumor-inducing (Ti) Plasmids

식물종양유도 플라스미드의 분리 및 동정

  • 음진성 (고려대학교 이과대학 생물학과) ;
  • 김계현 (고려대학교 이과대학 생물학과) ;
  • 심웅섭 (고려대학교 이과대학 생물학과)
  • Published : 1986.03.01


Tumor-inducing (Ti) plasmid which may be used in genetic engineering of higher plants, was isolated from Agrobacterium tumefaciens in Korea. Among the 7 strains of A. tumefaciens isolated from various regions in Korea, KU12, KU13, KU14, and KU49 strains were confirmed to contain plasmid. The isolated Ti plasmids were digested with 4 kinds of restriction enzymes, respectively. According to the result of the cleavage patterns, we confirmed that these plasmids in the KU12, KU13, KU14 and KU49 strains are different.