Genetic Recombination by Protoplast Fusion of Cellulomonas sp CS 1-1

원형질체 융합법에 의한 cellulomonas sp. CS 1-1의 유전자 재조합

  • 김병홍 (한국과학기술원 생물공학부 미생물연구실) ;
  • 이학주 (한국과학기술원 생물공학부 미생물연구실)
  • Published : 1985.12.01


Autoxotrophic mutants of a cellulolytic baterium Cellulomonas sp. CS 1-1 were grown at $30^{\circ}C$ for 6hr using a complete medium containing 0.5M sucrose and for another 90 min after addition of 0.3 U/ml penicillin G, and were protoplasted by 0.2mg/ml lysozyme for 2hr. Prototrophic recombinants were obtained at the rates of $10^{-3}$ to $10^{-5}$by fusing the protoplasts in the presence of 40% polyethyleneglycol3350. Nystatin could be used to eliminate fungal contamination during the regeneration of the plotaplasts.