Electron Microscopic Observation on Protoplast Fusion of Coryneform Bacteria

Coryne형 세균의 원형질체 융합에 관한 전자현미경적 관찰

  • Park, Chung (Department of Agricultural Chemistry, Korea University) ;
  • Lim, Bun-Sam (Department of Agricultural Chemistry, Korea University) ;
  • Chun, Moon-Jin (Department of Agricultural Chemistry, Korea University) ;
  • Kim, Woo-Kap (Department of Biology, Korea University)
  • 박청 (고려대학교 농화학과) ;
  • 임번삼 (고려대학교 농화학과) ;
  • 전문진 (고려대학교 농화학과) ;
  • 김우갑 (고려대학교 생물학과)
  • Published : 1985.12.01


Overall procedure of cell fusion between Brevibacterium flavum and Corynebacterium glutamicum was morphologically observed by transmission electron microscopy. Protoplasts formed by treatment of cells with penicillin G and lysozyme in order were released through the pores generated on a certain region of cell walls to be spherical form. When two different protoplasts were met, cell wall and membrane in the contact zone was disappeared and followed by the mutual exchange of cytoplasmic and/or chromosomal materials. Cell xall regeneration speed of the protoplasts fused was slower than that of the non-fused, whereas the size of the former was confirmed as bigger than that of the latter.