Purification and Characterization of the Regulatory Substance of Furfural Biodegradation in Pseudomonas fluorescens

Pseudomonas fluorescens에 의한 Furfural의 분해대사 조절물질에 관하여

  • 이병웅 (서울대학교 자연과학대학 미생물학과) ;
  • 유병설 (서울대학교 자연과학대학 미생물학과) ;
  • 이계준 (서울대학교 자연과학대학 미생물학과) ;
  • 하영칠 (서울대학교 자연과학대학 미생물학과)
  • Published : 1985.12.01

Abstract

The objectives of this study were to isolate and identify ninhydrin positive substande(s) produced in the culture broth of Pseudomonas fluorescens. It was found that the NPS could stimulate bioconversion of furfural into furoic acid. In order to isolate the NPS from the culture broth, cell free filtrate was subjected to ion-exchange chromatography, gel-permeation and finally to cellulose column chromatography. The purified NPS was white amorphous power and very soluble in water, slightly soluble in methanol and very insoluble in organic solvents. UV, and IR absorption spectra. $^H$ and $^{13}C-NMR$ were measured in order to identify the chemical structure of the NPS.

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