Study of Inulase from Kluyveromyces marxianus

Kluyveromyces marxianus가 분비하는 inulase에 대한 연구

  • 유연우 (아주대학교 공과대학 생물공학과) ;
  • 김신제 (아주대학교 공과대학 생물공학과) ;
  • 김수일 (서울대학교 농과대학 농화학과)
  • Published : 1984.03.31

Abstract

Kluyveromyces marxinus LG contains an inulase activity which is inducible by growth on inulin. The highest enzyme activity obtained at the initial stage of stationary phase of growth curve. The partially purified inulase hydrolyzed inulin, sucrose and raffinose. This enzyme showed the maximal activity at pH 4.0 and temperature of $55^{\circ}C$ for inulin and sucrose. It was also markedly inhibited by $Hg^{++}\;and\;Ag^{+}$ but not EDTA. This inulase was charaterized as a undo type which contains invertase activity and Km values for inulin and sucrose were $1.2{\times}10^{-2}M\;and\;1.3{\times}10^{-4}M$, respectively.

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