Gibberellin-like Activities Produced by mycorrhizal fungus, Pisolithus tinctorius

균근균(菌根菌), Pisolithus tinctorius가 생산(生産)하는 Gibberellin양(樣) 활성(活性)

  • Park, Keun-Hyung (Department of Food Science and Technology, College of Agriculture, Chonnam National University) ;
  • Kang, Sung-Hoon (Department of Food Science and Technology, College of Agriculture, Chonnam National University) ;
  • Kim, Dong-Youn (Department of Food Science and Technology, College of Agriculture, Chonnam National University) ;
  • Kim, Kwan (Department of Food Science and Technology, College of Agriculture, Chonnam National University) ;
  • Rhee, Chong-Ouk (Department of Food Science and Technology, College of Agriculture, Chonnam National University) ;
  • Jung, Ji-Heun (Department of Food Science and Technology, College of Agriculture, Chonnam National University)
  • 박근형 (전남대학교 농과대학 식품가공학과) ;
  • 강성훈 (전남대학교 농과대학 식품가공학과) ;
  • 김동연 (전남대학교 농과대학 식품가공학과) ;
  • 김관 (전남대학교 농과대학 식품가공학과) ;
  • 이종욱 (전남대학교 농과대학 식품가공학과) ;
  • 정지흔 (전남대학교 농과대학 식품가공학과)
  • Published : 1984.03.31

Abstract

Experiments on the GA production ability by ectomycorrhizal fungus, Pisolithus tinctorius was carried out to investigate specific physiological phenomena of growth increase in host plants by formation of mycorrhizae, The culture extract of P. tinctorius was purified by solvent fractionation, sephadex LH-20 chromatography, silica gel partition chromatography and TLC, successively. GA activities in the purified GA fractions were monitored by micro-drop bioassay using dwarf rice seedlings, 'Tan-ginbozu'. $30{\sim}60%$ EtOAc election fractions of silica gel pardon chromatography and the zone of Rf $0.1{\sim}0.4,\;0.6{\sim}0.8$ of TLC exhibited the GA-like activities. The GA activities were increased with the more treated amount of culture extracts. This activity in 100ml of culture solution was equivalent to 0.1ng of $GA_3$.

Keywords