Studies on the Production of Gibberellic acid

지베렐린 생산에 관한 연구

  • 이영선 (한국과학기술원 미생물연구실) ;
  • 손형진 (한국과학기술원 미생물연구실) ;
  • 김익환 (한국과학기술원 미생물연구실) ;
  • 민태익 (한국과학기술원 미생물연구실)
  • Published : 1983.09.01


By the treatment of Gibberella fujkuroi I-892 with mutagen such as UV light and N-methyl-N'-nitro-N-nitrosoguanidine, a mutant G. fujkuroi G-471 was selected as the highest producer of gibberellic acid among 800 mutant strains. It showed 30% increase of production yield compared with that of the parent strain. At optimum medium composition (saccharose 1.0%, ammonium tartarate 50mM, malt extract 1.0% KH$_2$PO$_4$ 0.5%, MgSO$_4$0.5%, FeSO$_4$0.0002%, trace element sol.0.002% (v/v), the yield of submerged culture increased by 30% after 7 days culture at 24$^{\circ}C$ (253mg/$\ell$). In submerged culture, the initial pH showed much effects on the increase of gibberellic acid production. The highest yield of the production was attained with pH adjustment to 4.0 at the initial stage of fermention.