Transformation of Bacillus Subtilis by Streptomyces bobili R-Plasmid DNA

Streptomyces bobili의 R-Plasmid. DNA에 의한 Bacillus subtilis의 Transformation

  • 김상달 (한국인삼연초연구소) ;
  • 도재호 (한국인삼연초연구소)
  • Published : 1983.09.01

Abstract

The penicillin resistant plasmid DNA was prepared from Streptomyces bobili YS-40, producing penicillinase, by the phenol extraction method and introduced into Bocillus subtilis IAM 12118 by the transformation procedure of Mahler method. The optimal pH and temperature on the transformation was 7.0, 3$0^{\circ}C$ respectively. Above 20 minutes contact of plasmid DNA and recipient cell was shown the high transformation frequency. The transformant of penicillin resistance was proportionally increased as increase of the DNA concentration. The addition of lysine in transformation system increased the transformation frequency about 6-fold and the addition of the chloramphenicol did not affect the transformation frequency.

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