A Study on Physiological conditions for hydrogen evolution by Rhodopseudomonass phaeroides K-7

Rhodopseudomonas sphaeroides K-7에 의한 수소생성에 미치는 생리적 조건에 관한 연구

  • Bae, Moo (Department of Biology, Ewha Women's University) ;
  • Lee, Jeong-Kug (Korea Advanced Institute of Science and Technology)
  • 배무 (이화여자대학교 자연대학 생물학과) ;
  • 이정국 (한국과학기술원 응용미생물연구실)
  • Published : 1983.09.01


Physiological conditions for hydrogen evolution by Rhodopseudomonas sphaeroides K-7 are examined. Larger amount of molecular hydrogen was evolved at $30^{\circ}C$, pH6.8-7.0 under anaerobically illuminated condition of about 12, 000 lux by the organism. The heighest rates of hydrogen evolution were observed in the culture with the organic acids such as acetate, DL-lactate or DL-malate in media containing L-glutamate as a nitrogen source. Hydrogen was also evolved from glucose with the rate of $178.9\;{\mu}l/hr/mg$ cells (dry weight). When glucose was adopted a a sole carbon source, however, conside erable time lag of about 20hours was required for hydrogen evolution. The resting cells stored at $30^{\circ}C$ under argon maintained the rate of hydrogen evolution in nearly about 90% of initial one even 40 days of storation.