Detection of Proteins from Porcine Follicular Fluid and Their Effect on the Maturation of Mouse Oocytes in vitro

돼지 여포액내(慮胞液內) 단백질(蛋白質)의 검출(檢出)과 배양중(培養中)인 생쥐란자(卵子)의 성숙(成熟)에 미치는 그의 영향에 관하여

  • Bae, In-Ha (Sungshin Women's College) ;
  • Hwang, Sung-Yun (Department of Zoology, College of Natural Sciences, Seoul National University) ;
  • Chung, Soon-O (College of Medicine, Yonsei University) ;
  • Cho, Wan-Kyoo (Department of Zoology, College of Natural Sciences, Seoul National University)
  • 배인하 (성신여자대학) ;
  • 황성윤 (서울대학교 자연과학대학 동물학과) ;
  • 정순오 (연세대학교의과대학) ;
  • 조완규 (서울대학교 자연과학대학 동물학과)
  • Published : 1981.05.31

Abstract

It has been already suggested that specific macromolecules in follicular fluid produced by granulosa cells may play a role in suppressing further meiotic maturation of the oocytes. In general, the search for specific macromolecules in follicular fluid using immunological methods has not been rewarding. These studies were designed, by applying more effective immunological methods than conventionally employed, (l) to identify whether some unknown macromolecules are present in the porcine follicular fluid or not, and (2) to clarify the relationship between the oocytes and the specific macromolecules in the follicular fluid. The results obtained were as follows; (1) porcine follicular fluid contained two specific proteins, which were not present in pig plasma and serum. (2) each of two proteins showed electrophoretically fast alpha-globulin and beta-globulin mobilities. (3) these proteins seemed to have inhibitory effect on the maturation of mouse oocytes in vitro. From these results, it can be assumed that pig follicular fluid contains specific proteins which seem to be intra-follicular inhibitor(s) of oocyte maturation.

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