Screening method of varietal resistance to planthoppers labeled with radioisotope $^{32}P$ ( I )

방사성동위원소 $^{32}P$를 이용한 멸구 류에 대한 품종저항성 검정방법에 관한 연구(I)

  • Lee J. O. (Institute of Agricultural Sciences, ORD) ;
  • Kim Y. H. (Institute of Agricultural Sciences, ORD) ;
  • Park J. S. (Institute of Agricultural Sciences, ORD) ;
  • Seok S. J. (Institute of Agricultural Sciences, ORD) ;
  • Goh H. G. (Institute of Agricultural Sciences, ORD)
  • 이정운 (농촌진흥청 농업기술연구소) ;
  • 김용헌 (농촌진흥청 농업기술연구소) ;
  • 박중수 (농촌진흥청 농업기술연구소) ;
  • 석순종 (농촌진흥청 농업기술연구소) ;
  • 고현관 (농촌진흥청 농업기술연구소)
  • Published : 1981.06.01

Abstract

The screening method of varietal resistance on the plant hoppers has generally been evaluated as a reaction of plant after infesting insects. However, feeding amount of insects to the varieties was investigated in this experiment. The new method using isotope $^{32}P$ for rice varietal resistance to plant hoppers was carried out through the following method. Insects tested were caged for a few hours on the plants which had absorbed $^{32}P$ solution in small vials for $24\~48\;hours$. After feeding, insects were killed in the refrigerator with formalin solution, and then were measured by the feeding amount as a count per minute (CPM) with the G.M. Counter. The results obtained were summarized as follows; 1. The apparatus of Type D(Fig. 2) was most effective and the safest among four others. 2. The optimum amount of $H_3PO_4$ solution was found to be $2\~3m1$. 3. Radioactivity of $0.7\mu\;Ci.\;^{32}P$ was sufficient to check varietal difference of feeding amount by the brown planthopper. 4. Radioisotope was found from the body of insects but not in the cuticular layer nymphs cast off.

Keywords