Studies on the Fermentative Production of Inosine 5'-monophosphate by Microorganisms. (Part II) Effects of Carbon Source and Purine Base on Inosine 5'-monophosphate Accumulation by a Mutant of Brevibacterium ammoniagenes

미생물에 의한 5'-이노신산의 생산에 관한 연구 (제 2보) Brevibacterium ammoniagenes 이변주에 의한 5'-이노신산의 생성에 미치는 탄소원과 Purine염기의 영향

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  • Hiroshi Iizuka ;
  • 고중환 (제일제당주식회사 식품연구소) ;
  • 공운영 (제일제당주식회사 식품연구소) ;
  • 손충홍 (제일제당주식회사 식품연구소) ;
  • 배종찬 (제일제당주식회사 식품연구소) ;
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  • 유주현 (연세대학교 식품공학과)
  • Published : 1981.03.01


The effect of growth and the carbon sources including the molar ratio of fructose to glucose was studied for the maximization of inosine-5'-monophosphate (5'-IMP) production from Brebibacterium ammoniagenes D-21530. According to experimental results, fructose was more efficient to 5'-IMP accumulation than glucose, while the latter was better for the cell growth than the former. To synchronously use glucose and fructose as carbon source is to optimally control the cell growth and maximum production of 5'-IMP without change of other conditions. The optimal weight percent of fructose to sum of glucose and fructose was 20~40%, and the productivity improvement over the utilization of fructose was about 40%. And also the optimality of purine base such as adenine and guanine were considered. The optimal concentrations of adenine and guanine were near 50㎎/l.