Studies on Microbial Penicillin Amidase ( I ) Optimization of the Enzyme Production from Escherichia coli

미생물 페니실린 아미다제에 관한 연구 (I) E. coli로부터 효소생산 조건의 최적화

  • Kim, Bong-Hee (Biotechnology Research Department, Korea Institute of Science and Technology) ;
  • Seong, Baik-Lin (Biotechnology Research Department, Korea Institute of Science and Technology) ;
  • Mheen, Tae-Iek (Biotechnology Research Department, Korea Institute of Science and Technology) ;
  • Moon H. Ban (Biotechnology Research Department, Korea Institute of Science and Technology)
  • Published : 1981.03.01

Abstract

To maximize the production of penicillin amidase from Estherichia coli (ATCC 9637), the media composition and several factors affecting the engyme production during fermentation were studied. The optimal media composition was found to be; 3.5% tryptone, 1.5% monosodium glutamate and 0.5% yeast extract. The addition of 0.15% phenylacetic acid as an enzyme inducer at the initial stage of cultivation increased the engyme productivity about 5 fold. It was found that the engyme activity reached maximum within 16hr of cultivation. The maximum production of the enzyme obtained was about 102.5 units/l broth under the optimized condition. The enzyme production was markedly increased by the optimization as compared with those previously reported.

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