Studies on the Production of Gluconic Acid by Resting Cell System of Aspergillus niger

Aspergillus niger의 휴지균체에 의한 Gluconic Acid생성에 관한 연구

  • 정지관 ((주)종근당, 중앙연구소) ;
  • 양호석 ((주)종근당, 중앙연구소) ;
  • 신규철 ((주)종근당, 중앙연구소) ;
  • 양한철 (고려대학교 농과대학 식품공학과)
  • Published : 1981.03.01

Abstract

The production of gluconic acid from glucose by the resting cell system of Aspergillus niger was studied. It was found that the conversion products from glucose by the resting cell system were markedly influenced by the pH, temperature, substrate concentration, aeration, metal ions, cultivation time and storage conditions of the resting cells. Conversion products were identified as gluconic acid by the thin layer chromatography and infrared spectrophotometry. These conversions were greatly stimulated by addition of $Mg^{++}$, and S $n^{++}$, but showed inhibitory effects by C $u^{++}$, H $g^{++}$, C $d^{++}$, A $g^{+}$ and cyanide. For the optimum cell storage, it was effective to be kept at -$25^{\circ}C$ in 0.05M phosphate buffer solution of pH 7.0. The gluconic acid production by the resting cell system was more effective than those of the fermentation with respect to cultivation time, yield, recovery and re-use of the cell.l.l.l.l.l.l.

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