Studies on the Production of Guanosine-5'-monophosphate by Microorganism. (Part III) Studies on the Crystallization of 5'-GMP. 2Na.

미생물에 의한 5'-GMP의 생산에 관한 연구 (제3보) 5'-GMP의 결정화에 관한 연구

  • 이계하 (제일제당(주) 식품연구소) ;
  • 문화식 (제일제당(주) 식품연구소) ;
  • 이희인 (제일제당(주) 식품연구소) ;
  • 배종찬 (제일제당(주) 식품연구소) ;
  • 류주현 (연세대학교 식품공학과)
  • Published : 1981.03.01


Crystallization conditions of disodium guanosine-5'-monophosphate (5'-GMP. 2Na) were studied. The solubility of 5'-GMP. 2Na was decreased by addition of methanol and the optimum condition was as follows. The crystallization was carried out at 45$^{\circ}C$ with agitation rate of 160-200 rpm., which is Reynold's No. of 25, 000-32, 000. When concentration of methanol was 7.5%~10.0%, the 5'-GMP. 2Na was easily crystallized by addition of crystal seed.