Formation of Protoplast from Trichoderma koningii

Trichoderma koningii의 Protoplast 생성에 관하여

  • 조남진 (서울대학교 자연과학대학 미생물학과) ;
  • 이영하 (서울대학교 자연과학대학 미생물학과) ;
  • 홍순우 (서울대학교 자연과학대학 미생물학과)
  • Published : 1981.12.01


Protoplast production from Trichoderma koningii ATCC 26113 was investigated for the purpose of doing basic and applied researches by protoplast fusion of the cellulolytic filamentous fungus. High yields of protoplast were obtained by using the 18hr. old mycelia treated with the lytic enzyme Driselase of Kyowa Fermentation Co., Japan, in 0.6M $MgSO_4\;or\;(NH_4)_2SO_4$ as osmotic stabilizers. The optimum temeprature of mycelial digestion was about $28^{\circ}C$ and the number of protoplast increased rapidly after 3hr. digestion. Protoplasts produced at different periods showed distinct morphological heterogeneities in the whole size and the size of vacuole.