Studies on the Callus

마늘의 Callus 배양에 관한 연구

  • 장무웅 (영남대학교 생물학과) ;
  • 이갑랑 (영남대학교 식품영양학과) ;
  • 조수열 (영남대학교 식품영양학과) ;
  • 정희돈 (영남대학교 원예학과)
  • Published : 1980.06.01


The experiment was conducted to culture callus tissue induced from foliage leaf of garlic bulb for the production of virus-free stocks and for the reduction of expenses for seeds, The following results were reached. 1. Linsmaier-Skoog basal medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) $10^{-5}M$ and benzyladenine $10^{-5}M$ showed the most effective for the induction for the induction of garlic callus. 2. The growth rate of callus was the highest in Linsmaier-Skoog basal medium containing kinetin $10^{-6}M\;and\;2,4-D\;10^{-6}M$ 3. The results of periodical assay of virus concentration in callus tissues showed that virus was almost eliminated by repeated transfer of translucent and soft tissue for eight generations. 4. When virus-free garlic callus tissues were transfered to Murashige-Skoog basal medium containing kinetin $10^{-5}M$ and naphthaleneacetic acid $5\times10^{-6}M$, the tissues were redifferentiated and formed plantlet.