- Volume 8 Issue 3
Formation of D-Glucose Isomerase by Streptomyces sp.
Streptomyces sp.에 의한 포도당 이성화효소의 생성
- Rhee, In-Koo (Department of Agricultural Chemistry, College of Agriculture, Kyungbook National University) ;
- Seu, Jung-Hwn (Department of Agricultural Chemistry, College of Agriculture, Kyungbook National University)
- Published : 1980.09.01
A source of D-xylose was required for the enhanced production of D-glucose isomerase of Streptomyces sp. strain K-17. D-glucose supported the luxuriant growth of the organism as well as D-xylose, but D-glucose isomerase activity was hardly detected in the D-glucose-grown cells. When the D-glucose-grown cells were incubated aerobically for a few hours in 0.5% xylose solution in 0.05 M phosphate buffer, pH 7.0, it was found that inductive formation of D-glucose isomerase occurred in the cells without multiplication. In the non-growth phase of cells the inductive formation of D-glucose isomerase occurred because a source of nitrogen for the synthesis of enzymes was obtained from turnover of protein accumulated in cells. D-ribose, L-arabinose, D-glucose, D-mannose, citrate, succinate and tartrate could not induce the formation of D-glucose isomerase, but D-xylose could induce. Inductinn of D-glucose isomerase was repressed by D-glucose and its catabolites : glycerol, succinate and citrate. Inductive formation of the enzymes in the non-growth phase was stimulated by