Radioisotope Labelling Method of Pine Gall Midge (Thecodiplosis japonensis UCHIDA et INOUYE)

솔잎혹파리에 대한 방사선동위원소 표식방법에 관한 연구

  • Kwon S. H. (Korea Atomic Energy Research Institute, Radiation Breeding Laboratory) ;
  • Chung K. H. (Korea Atomic Energy Research Institute, Radiation Breeding Laboratory) ;
  • Ryu J. (Korea Atomic Energy Research Institute, Radiation Breeding Laboratory)
  • 권신한 (한국원자력연구소 방사선육종학연구실) ;
  • 정규회 (한국원자력연구소 방사선육종학연구실) ;
  • 유준 (한국원자력연구소 방사선육종학연구실)
  • Published : 1978.09.01

Abstract

This experiment was carried out to develope a technique for labelling pine gall midge with ·radioactive phosphorus and calcium. This technique evolved would help to obtain basic information on the appropriate concentration of $^{32}P\; and\; ^{45}Ca$ in tagging and determination of adult flight ranges which considered as one of the factors in dissemination. 1. Labelling at the larval stage of pine gall midge by injection of $^{45}Ca$ into pine tree trunk appeared not to be effective for tagging the adults because radioactivity was gradually decreased to background by emergence. 2. The radioactivities of larvae in the galls gradually increased in September but from October 1 the activity was gradually decreased for 10 days then equilibrium was maintained thereafter. The results seem to indicate that larval diet termination occured early October. 3. A maximum level of radiation was detected at 3 weeks after $^{32}P$ injection and thereafter it was gradually reduced. This result implies that $^{32}P$ is not appropriate radioisotope in labelling pine gall midge through trunk injection method. 4. After washing in running water for 10 minutes, the optimum$^{32}P$ concentration for treated overwintered larvae and pupae was found to be 0.5 micro curie per ml. for 30 minutes dipping.

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