- Volume 17 Issue 1 Serial No. 34
Purification and Serology of Cucumber Mosaic Virus
오이모자익 바이러스의 순화와 항혈청 제조
- Lee S. H. (Dept. of plant Pathology, Institute of Agricultural Sciences, ORD) ;
- Lee K. W. (Dept. of plant Pathology, Institute of Agricultural Sciences, ORD) ;
- Chung B. J. (Dept. of plant Pathology, Institute of Agricultural Sciences, ORD)
- Published : 1978.03.01
Purely isolated cucumber mosaic virus (CMV) was multiplied in Nicotiana tabacum, Ky-57 and the virus was purified by the modified method that was developed through this study. The concentration of purified CMV was 24.25 mg/ml. The purified virus, mixed with acomplet adjuvant (1: 1) was injected into rabbits intramuscularly. Two injections at 10 day interval was enough to produce a good quality antiserum. The titer of the antiserum was 1/1280 when determined by agar gell-diffusion test. The produced antisera will be used to faciliate the detection of CMV infected vegetables and other crops.