Purification and Serology of Cucumber Mosaic Virus

오이모자익 바이러스의 순화와 항혈청 제조

  • Lee S. H. (Dept. of plant Pathology, Institute of Agricultural Sciences, ORD) ;
  • Lee K. W. (Dept. of plant Pathology, Institute of Agricultural Sciences, ORD) ;
  • Chung B. J. (Dept. of plant Pathology, Institute of Agricultural Sciences, ORD)
  • 이순형 (농촌진흥청 농기연 병리과) ;
  • 이기운 (농촌진흥청 농기연 병리과) ;
  • 정봉조 (농촌진흥청 농기연 병리과)
  • Published : 1978.03.01

Abstract

Purely isolated cucumber mosaic virus (CMV) was multiplied in Nicotiana tabacum, Ky-57 and the virus was purified by the modified method that was developed through this study. The concentration of purified CMV was 24.25 mg/ml. The purified virus, mixed with acomplet adjuvant (1: 1) was injected into rabbits intramuscularly. Two injections at 10 day interval was enough to produce a good quality antiserum. The titer of the antiserum was 1/1280 when determined by agar gell-diffusion test. The produced antisera will be used to faciliate the detection of CMV infected vegetables and other crops.

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