Studies on the Microbial Glucose Isomerase Part 2. Culture Conditions of Streptomytes sp. K-14 in Producing Glucose Isomerase

미생물의 포도당 이성화효소에 관한 연구 (제2보) Streptomyces sp. K-14 균주의 배양특성에 하여

  • Tai Wha Chung (Applied Biochemistry Labo., Korea Institute of Science & Technology) ;
  • Moon H. Han (Applied Biochemistry Labo., Korea Institute of Science & Technology)
  • Published : 1976.12.01

Abstract

Cultural characteristics of a strain of Streptomyces sp. K-14 (KFCC 35051) producing glucose isomerase were demonstrated. The glucose isomerase was produced when the strain was grown in the medium containing pure xylan or xylan.containing materials such as wheat bran or com cob. The optimum condition was attained in a culture medium composed of 3 % wheat bran or com cob, 2 % com steep liquor, 0.1% $MgSO_4$$7H_2O$ and 0.012 % $CoSO_4$$7H_2O$ for the production of the glucose isomera,e. The production of the enzyme reached to a maximum level when the strain was cultured for 40 hrs $30^{\circ}C$ and pH 7.0.

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