Studies on the effects of culture filtrates of sesame-wilt organism(Fusarium oxysprum f. vasinfectum) on the germination of sesame seeds and the growth of sesame seedlings

참깨$\cdot$시들음병균(Fusarium oxysporum)의 배양여액이 참깨의 종자발아 및 유묘기의 생육에 미치는 영향

  • Published : 1962.12.01

Abstract

1) The purpose of the present study is to investigate the effects of culture filtrates of Fusarium oxysporum f. vasinfectum which is known to produce fusaric acid (wilt toxin) on the germination of sesame seeds and the growth of sesame seedlings. 2) Culture filtrates of Fusarium oxysporum 1. vasinfectum used in this study strongly or weakly inhibited the germination and bring about necrosis accompanying black discoloration of sesame seeds. 3) Varietal difference of sesame in the germination response on the culture filtrates of Fnsarium oxysporum f. vasinfectum is not shown in this study. 4) This study reveals that differential five strains of Fnsarium oxysoprum f. vasinfectum used in this study differ greatly in the toxicity of culture filtrates inhibiting the germination of sesame seeds. 5) In the seedling bed added with culture filtrates of Fnsarium oxysporum f. vasinfectum, the growth of shoot as well as root system of sesame seedlings are notably inhibited and necrotic black discoloration appear on both shoot and root system. But in the seedling beds added with weaker concentration of culture filtrates $/(10\%)$ the growth of shoot is slightly promoted. 6) In culture of sesame seedlings with Knop's solution containing 1 to 3 per cent culture filtrates, the growth of shoot as well as root system are slightly retarded" and till the time of development of the third leaves the whole stem and leaf petiole tissue are weakened so that they become thread like accompanying brown discoloration, interveinal light brown area appear in the second leaves, and the third leaves curl from both sides towards the middle with necrotic brown discoloration, especially symptoms of injury on the third leaves are nearly similar that of the leaves of wilted sesame in the field. 7) A pararell relationship is not found between toxicity of culture filtrates and pathogenicity of five differential strains of Fnsarium oxysporum f. vasinfectum used in this study.

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