Corn Straw as Substrate for Producing Ethanol by Solid-State-Fermentation

  • Dong, Yong-Sheng (College of Biotechnology, Tianjin University of Science & Tecnology) ;
  • Qiao, Chang-Sheng (College of Biotechnology, Tianjin University of Science & Tecnology) ;
  • Wang, Rui-Ming (College of Biotechnology, Tianjin University of Science & Tecnology) ;
  • Wang, Li-Yan (College of Biotechnology, Tianjin University of Science & Tecnology) ;
  • Jia, Shi-Ru (College of Biotechnology, Tianjin University of Science & Tecnology)
  • Published : 2005.06.30

Abstract

The technology of Solid-State-Fermentation (SSF) with corn straw by Pichia ohmeri T021 was studied in this article. After being crushed, the corn straw powder was added into vitriol solutions to hydrolysis, which the quality proportion of corn straw: water: vitriol (98%) is 20:80:1. The mixtures was incubated at 120$^{\circ}C$ for 1 hour, and the hydrolysis rate reached 19%. Following, the mixture was adjusted to pH 4.5 by sodium carbonate and added cellulase (25IFPU/g). The hydrolysis rate reached 15% after the mixture was incubated at 50$^{\circ}C$ for 25h. The mixture which hydrolysed by vitriol was inoculated by Pichia ohmeri T021 (5${\times}10^7$cell/g) and added cellulase (25 IFPU/g) at the same time. The ethanol yield reached 2.99g per 100 gram substrate after the fermenting grains was incubated at 33$^{\circ}C$, pH 4.5 for 5 days.

Keywords

Solid-State-Fermentation;Corn Straw;Ethanol