In this paper, the multi-channel high speed data acquisition system is implemented. This high speed signal processing system for 3-D image display is applicable to the manipulation of a medical image processing, multimedia data and various fields of digital image processing. In order to convert the analog signal into digital one, A/D conversion circuit is designed. PCI interface method is designed and implemented, which is capable of transmission a large amount of data to computer. In order to, especially, channel extendibility of images acquisition, bus communication method is selected. By using this bus method, we can interface each module effectively. In this paper, 32-channel A/D conversion and PCI interface system for 3-dimensional and real-time display of the retina image is developed. The 32-channel image acquisition system and high speed data transmission system developed in this paper is applicable to not only medical image processing as 3-D representation of retina image but also various fields of industrial image processing in which the multi-point realtime image acquisition system is needed.