IN VITRO AND IN VIVO EVALUATION OF THE GENOTOXIC EFFECT OF 2-BROMOPROPANE BY THE ALKALINE SINGLE-CELL GEL ELECTROPHORESIS(COMET) ASSAY

  • Kim, Soo-Jin (Center for Occupational Toxicology, Occupational Safety and Health Research Institute, Korea Occupational Safety & Health Agency) ;
  • Yu, Il-Je (Center for Occupational Toxicology, Occupational Safety and Health Research Institute, Korea Occupational Safety & Health Agency) ;
  • Lee, Yong-Mook (Center for Occupational Toxicology, Occupational Safety and Health Research Institute, Korea Occupational Safety & Health Agency) ;
  • Chung, Ho-Keun (Center for Occupational Toxicology, Occupational Safety and Health Research Institute, Korea Occupational Safety & Health Agency) ;
  • Maeng, Seung-Hee (Center for Occupational Toxicology, Occupational Safety and Health Research Institute, Korea Occupational Safety & Health Agency)
  • Published : 2002.11.01

Abstract

The alkaline single cell gel electrophoresis (comet) assay was used to clarify in vitro and in vivo genotoxicity of 2-bromopropane (2-BP). For in vitro studies, fresh medium containing 2-BP (2.50, 1.00, 0.50, 0.25, 0.10, 0.05, 0.01 mM, and vehicle control) were added in human lymphocytes.(omitted)