A study of in situ immobilization of lipase by using an LC column with aldehyde-silica stationary phase

  • 서우용 (명지대학교 환경생명공학과) ;
  • 홍선희 (명지대학교 환경생명공학과) ;
  • 이기세 (명지대학교 환경생명공학과)
  • Published : 2001.11.07

Abstract

The in situ immobilization of lipase in an LC column with aldehyde-silica stationary phase was optimized. The effects of feed composition, temperature, mobile phase pH and now rate on immobilization efficiency were investigated. Lipase activity after in situ immobilization was compared with free enzyme and batch immobilized enzyme.

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