The detection of Human Papillomavirus (HPV) by the polymerase chain reaction(PCR) in head and neck cancers

두경부암에서 중합효소 연쇄반응을 이용한 유두종 바이러스의 검출

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  • Richard E Hayden (Department of Otolaryngology Head & Neck Surgery, University of Pennsylvania) ;
  • David B Weiner (Department of Otolaryngology Head & Neck Surgery, University of Pennsylvania)
  • 김민식 (가톨릭대학교 의과대학 이비인후과학교실) ;
  • 조승호 (가톨릭대학교 의과대학 이비인후과학교실) ;
  • 서병도 (가톨릭대학교 의과대학 이비인후과학교실) ;
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  • Published : 1993.05.01

Abstract

Polymerase chain reaction is widely used as a powerful tool in modern molecular biology. As there is agreement that the HPV is an important factor in the head and neck cancers, the detection of HPV DNA sequence in the head and neck cancer tissue has been tried in several ways. We used the PCR to detect the E1 open reading frames of the HPV in paraffin-embedded tissue of the patients with the head neck cancers. Eleven of the fifty-four tested samples (30%) showed positive result. We have analysed the clinical courses and characteristics related with Human Papillomavirus in those patients.

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