Characterization of Phytoplasmal Disease Occurred on Floricultural Crops in Korea
  • * Journal title : Research in Plant Disease
  • * Volume 17, Issue 3,  2011, pp.265-271
  • * Publisher : Korean Society of Plant Pathology
  • * DOI : 10.5423/RPD.2011.17.3.265
 Title & Authors
Characterization of Phytoplasmal Disease Occurred on Floricultural Crops in Korea
Chung, Bong-Nam ; Jeong, Myeong-Il ; Choi, Gug-Sun
 Abstract
Seven phytoplasma diseases have been occurred on floricultural crops in Korea : Ph-ch1 and Ph-ch2 of chrysanthemum, Ph-lily of lily, petunia flat stem-Korean (PFS-K) of petunia, poinsettia branch inducing- Korean (PoiBI-K) of poinsettia, statis witches' broom-Korean (SWB-K) of statis and azalea witches broom (AWB). Classification of the seven phytoplasmal diseases based on 16S ribosomal RNA (rRNA) sequences showed that floricultural crop phytoplasma disease were widespread in order of aster yellow (AY), stolbur and X-disease in Korea. In phenotypic characters, the fasciation was occurred in both monocotyledon plant of lily and dicotyledon plants of petunia and poinsettia. Besides, the fascination was occurred in Ph-lily of stolbur, petunia PFS-K of AY and PoiBI-K of X-disease. This result indicated that phytoplasma classification based on 16S rRNA and symptoms are not consistently related. The comparison of 16S rRNA sequence of the seven floricultural crop phytoplasma with five tree phytoplasmal diseases of jujube witches' broom, paulownia witches' broom, wild jujube witches' broom, mulberry dwarf, golden rain phytoplasma occurred in Korea showed as high as 88.5-99.9% homology. Among them, especially mulberry dwarf showed the highest homology with the seven floricultural crop phytoplasms. Based on this result, floricultural crop phytoplasmas were assumed to be transmitted by insect vectors from tree phytoplasmas in Korea.
 Keywords
Chrysanthemum . Lily . Petunia . Poinsettia . Statis
 Language
Korean
 Cited by
 References
1.
Ahrens, U. and Seemüller, E. 1992. Detection of DNA of plant pathogenic mycoplasmalike organisms by a polymerase chain reaction that amplifies a sequence of the 16S rRNA gene. Phytopathology 82: 828-832.

2.
Appiano, A., D'Giovanni, G. and Conti, M. 1983. Ultractural observations on chrysanthemum plants infected by a mycoplasma-like organism. Giornale Botanico Italiano 117: 136-137.

3.
Bertaccini, A., Davis, R. E., Hammond, R. W., Bibio, M., Bellardi, M. G. and Lee, I. M. 1992. Sensitive detection of mycoplasmalike organisms in field collected and in vitro propagated plants of Brassica, Hydrangea and Chrysanthemum by polymerase chain reaction. Ann. Appl. Biol. 121: 593-599.

4.
Bertaccini, A., Davis, R. E. and Lee, I. M. 1990. Distinctions among mycoplasmalike organisms (MLOs) in Gladiolus, Ranunculus, Brassica, and Hydrangea through detection with nonradioactive cloned DNA probes. Phytopath. Medit. 29: 107-113.

5.
Bosco, D., Minucci, C., Boccardo, G. and Conti, M. 1997. Differential acquisition of chrysanthemum yellows phytoplasma by three leafhopper species. Entomol. Exp. Appl. 83: 219-224.

6.
Chung, B. N. and Choi, G. S. 2010. Occurrence of poinsettia stem flat disease caused by phytoplasma in Korea. Plant Dis. 94: 792

7.
Chung, B. N., Choi, G. S. Kim, H. R. and Choi, Y. M. 2001. Identification of aster yellows phytoplasma in Dendranthema grandiflorum. Plant Pathology J. 17: 57−61.

8.
Chung, B. N. and Huh, K. Y. 2008. Occurrence of petunia flattened stem caused by phytoplasma. Plant Pathology J. 24: 279−282.

9.
Chung, B. N., Huh, K. Y. and Jeong, M. I. 2005. First report on the witches' broom in annual statice (Limonium sinuatum) in Korea. Plant Pathology J. 21: 383−386.

10.
Chung, B. N. and Jeong, M. I. 2003. Detection and molecular characterization of a Stolbur phytoplasma in Lilium oriental hybrids. Plant Pathology J. 19: 106−110.

11.
Chung, B. N. and Kim, B. D. 2005. Two groups of phytoplasma from chrysanthemum (Dendranthema grandiflorum) distinguished by symptoms and 16S rRNA gene sequence in Korea. Plant Pathology 21: 132-136.

12.
Chung, B. N., Kim, J. S. and Jeong, M. I. 2007. Phytoplasma disease in field grown chrysanthemum (Dendranthema grandiflorum). HEB. 48: 81-84.

13.
Conti, M., D'Agostino, G., Casetta, A. and Mela, L. 1988. Some characteristics of chrysanthemum yellows disease. Acta Hort. 234: 129-136.

14.
Doi, Y., Teranaka, M., Yora, K. and Asuyama, H. 1967. Mycoplasma- or PLT-like microorganisms found in the phloem elements of plants infected with mulberry dwarf, potato witches' broom, aster yellows, or paulownia witches' broom. Ann. Phytopathol. Soc. Jpn. 33: 259-266.

15.
Gundersen, D. E. and Lee, I. M. 1996. Ultrasensitive detection of phytoplasmas by nested-PCR assays using two universal primer pairs. Phytopath. Medit. 35: 144-151.

16.
Gundersen, D. E., Lee, I. M. Rehner, S. A., Davis, R. E. and Kingsbury, D. T. 1994. Phylogeny of mycoplasma like organisms (phytoplasmas): a basis for their classification. J. Bacteriol. 176: 5244-5254.

17.
He, F. T., Lee, I. M., Hammond, R. W. and Zhao, Y. 1998. Preliminary investigation of the role of protein kinase gene expression in the induction of phyllody symptoms on plants infected with phytoplasmas. 12th Int. Organ. Mycoplasmol. Conf. p. 184.

18.
Hiruki, C., Rong, X. D. and Deng, S. J. 1994. Hydrangea virescence, A disease associated with mycoplasma like organisms in Canada. Acta Hort. 377: 325-333.

19.
Hopkins, D. I. 1977. Diseases caused by leafhopper-borne rickettsia like bacteria. Ann. Rev. Phytopath. 17: 277-294.

20.
Kamin´ska, M. and Korbin, M. 2002. Detection of phytoplasma infection in Lilium sp. plants. Acta Hort. 568: 227-236.

21.
Kamin´ska, M. and Malinowski, T. 1996. Etiology of yellows and witches' broom symptoms in some ornamental plants. Acta Hort. 432: 96-106.

22.
Kaminska, M. and Sliwa, H. 2004. First report of phytoplasma belong to apple proliferation group in roses in Poland. Plant Dis. 88: 1283.

23.
Khadhair, A. H., Kawchuk, L. M. Taillon, R. C. and Botar, G. 1998. Detection and molecular characterization of an aster yellows phytoplasma in parsley. Can. J. Bot. 20: 55-61.

24.
Klingaman, G. 2008. Planting time fasciated plants result of natural mutation. Three Rivers, February, 114.

25.
Kunkel, L. O. 1926. Incubation period of aster yellows in its insect host. Phytopathology 16: 67. (Abstract)

26.
Lee, I. M., Davis, R. E. and Gundersen-Rindal, D. E. 2000. Phytoplasma: Phytopathogenic mollicutes. Annu. Rev. Microbiol. 54: 221-255.

27.
Lee, I. M., Dawn, E., Gundersen-Rindal, P. E., Robert, E. D. and Irena, M. B. 1998. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int. J. Syst. Bacteriol. 48: 1153-1169.

28.
Lee, J. T. 2004. Phytoplasmal disease in Korea. Junghaeng-Sa. Korea.

29.
Lee, I. M., Hammond, R. W. Davis, R. E. and Gundersen, D. E. 1993. Universal amplification and analysis of pathogen 16S rDNA for classification and identification of mycoplasma like organisms. Phytopathology 83: 834-842.

30.
Lee, I. M., Klopmeyer, M., Bartoszyk, I. M., Gundersen-Rindal, D. E., Chou, T. S., Thomson, K. L. and Eisenreich, R. 1997. Phytoplasma induced free-branching in commercial poinsettia cultivars. Nature Biotechnol. 15: 178-182.

31.
McCoy, R. E., Caudwell, A., Chang, C. J., Chen, T. A., Chiykowski, L. N., Cousin, M. T., Dale, J. L., de Leeuw, G. T. N., Golino, D. A., Hackett, K. J., Kirkpatrick, B. C., Marwitz, R., Petzold, H., Sinha, R. C., Sugiura, M., Whitecomb, R. F., Yang, I. L., Zhu, B. M. and Seemüller, E. 1989. Plant diseases associated with mycoplasma like organisms. In: The Mycoplasmas, vol. 5, ed. by R. F. Whitcomb and J. G. Tully, pp. 545-640. Academic Press, New York.

32.
Neimark, H. and Kirkpatrick, B. C. 1993. Isolation and characterization of full-length chromosomes from non-culturable plantpathogenic mycoplasma-like organisms. Mol. Microbiol. 7:21-28.

33.
Okuda, S., Prince, J. P., Davis, R. E., Dally, E. L., Lee, I. M., Mogen, B. and Kato, S. 1997. Two groups of phytoplasmas from Japan distinguished on the basis of amplification and restriction analysis of 16S rDNA. Plant Dis. 81: 301-305.

34.
Schaff, D., Lee, I. M., and Davis, R. E. 1992. Sensitive detection and identification of mycoplasma-like organisms in plants by polymerase chain reactions. Biochem. Biophys. Res. Commun. 186: 1503-1509.

35.
Schneider, B., Ahrens, U., Kirkpatrick, B. C. and Seemüller, E. 1993. Classification of plant pathogenic mycoplasma-like organisms using restriction-site analysis of PCR-amplified 16S rDNA. J. Gen. Microbiol. 139: 519-727.

36.
Schneider, B. and Seemüller, E. 1994. Studies on texonomic relationships of mycoplasmalike organisms by Southern blot analysis. J. Phytopathol. 141: 173-185.

37.
Seemuller, E., Schneider, B., Mürer, R., Ahrens, U., Daire, X., Kison, H., Lorenz, K. H., Firrao, G., Avinent, L., Sears, B. B. and Stackebrandet, E. 1994. Phylogenetic classification of phytopathogenic Mollicutes by sequence analysis of 16S ribosomal DNA. Int. J. Syst. Bacteriol. 44: 440-446.

38.
Smookler, M. and Dabush, S. 1974. Mycoplasma like bodies in tulips. Plant Dis. Rep. 58: 1142.

39.
Vorackova, Z. P., Fraova, J., Valova, P., Mertelik, J., Narail, M. and Nebesaova, J. 1998. Identification of phytoplasma infecting Lilium martagon in the Czech Republic. J. Phytopathol. 146: 609-612.

40.
Wakibe, H. and Guo, Y. H. 1998. Occurrence of static witches' broom disease transmitted by Macrosteles striifrons in Saga prefecture. Ann. Phytopathol. Soc. Jpn. 64: 383-384.

41.
Wei, W., Cai, H., Jiang, Y., Lee, I. M., Davis, R. E., Ding, Y., Yuan, E., Chen, H. and Zhao, Y. 2011. A new phytoplasma associated with little leaf disease in azalea: multilocus sequence characterization reveals a distinct lineage within the aster yellows phytoplasma group. Ann. Appl. Biol. 158: 318-330.

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